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plasmid hsv dyn hm4di  (Addgene inc)


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    Addgene inc plasmid hsv dyn hm4di
    Plasmid Hsv Dyn Hm4di, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+hsv+dyn+hm4di/HSV-DYN-hM4Di+(Plasmid+%2353327)/pmc11406995-195-20-28
    Average 92 stars, based on 7 article reviews
    plasmid hsv dyn hm4di - by Bioz Stars, 2026-11
    92/100 stars

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    Related Articles

    Amplification:

    Article Title: LIVE-CELL FLUORESCENCE MICROSCOPY OF HSV-1 CELLULAR EGRESS BY EXOCYTOSIS
    Article Snippet: .. The amplicon vector plasmid pCPD-HSV-N-EmGFP-DEST was constructed by the DNASU Plasmid Repository (Biodesign Institute, Arizona State University), as follows: The plasmid HSV-DYN-hM4Di was a gift from John Neumaier (Addgene plasmid # 53327) [ ]. ..

    Article Title: Herpes Simplex Virus 1 (HSV-1) Uses the Rab6 Post-Golgi Secretory Pathway For Viral Egress
    Article Snippet: .. The amplicon vector plasmid pCPD-HSV-N-EmGFP-DEST was constructed by the DNASU Plasmid Repository (Biodesign Institute, Arizona State University), as follows: The plasmid HSV-DYN-hM4Di was a gift from John Neumaier (Addgene plasmid # 53327) [ ]. ..

    Article Title: The Rab6 post-Golgi secretory pathway contributes to herpes simplex virus 1 (HSV-1) egress.
    Article Snippet: .. HSV-1 amplicon vector construction and propagation The amplicon vector plasmid pCPD-HSV-N-EmGFP-DEST was constructed by the DNASU Plasmid Repository (Biodesign Institute, Arizona State University), as follows: the plasmid HSV-DYN-hM4Di was a gift from John Neumaier (Addgene plasmid #53327) (47). ..

    Article Title: Individual herpes simplex virus 1 (HSV-1) particles exit by exocytosis and accumulate at preferential egress sites.
    Article Snippet: .. HSV-1 amplicon vector construction and propagation The amplicon vector plasmid pCPD-HSV-N-EmGFP-DEST was constructed by the DNASU Plasmid Repository (Biodesign Institute, Arizona State University) as follows: the plasmid HSV-DYN-hM4Di was a gift from John Neumaier (Addgene plasmid # 53327) (79). ..

    Article Title: The Rab6 post-Golgi secretory pathway contributes to herpes simplex virus 1 (HSV-1) egress
    Article Snippet: .. The amplicon vector plasmid pCPD-HSV-N-EmGFP-DEST was constructed by the DNASU Plasmid Repository (Biodesign Institute, Arizona State University), as follows: the plasmid HSV-DYN-hM4Di was a gift from John Neumaier (Addgene plasmid #53327) ( ). ..

    Plasmid Preparation:

    Article Title: LIVE-CELL FLUORESCENCE MICROSCOPY OF HSV-1 CELLULAR EGRESS BY EXOCYTOSIS
    Article Snippet: .. The amplicon vector plasmid pCPD-HSV-N-EmGFP-DEST was constructed by the DNASU Plasmid Repository (Biodesign Institute, Arizona State University), as follows: The plasmid HSV-DYN-hM4Di was a gift from John Neumaier (Addgene plasmid # 53327) [ ]. ..

    Article Title: Herpes Simplex Virus 1 (HSV-1) Uses the Rab6 Post-Golgi Secretory Pathway For Viral Egress
    Article Snippet: .. The amplicon vector plasmid pCPD-HSV-N-EmGFP-DEST was constructed by the DNASU Plasmid Repository (Biodesign Institute, Arizona State University), as follows: The plasmid HSV-DYN-hM4Di was a gift from John Neumaier (Addgene plasmid # 53327) [ ]. ..

    Article Title: The Rab6 post-Golgi secretory pathway contributes to herpes simplex virus 1 (HSV-1) egress.
    Article Snippet: .. HSV-1 amplicon vector construction and propagation The amplicon vector plasmid pCPD-HSV-N-EmGFP-DEST was constructed by the DNASU Plasmid Repository (Biodesign Institute, Arizona State University), as follows: the plasmid HSV-DYN-hM4Di was a gift from John Neumaier (Addgene plasmid #53327) (47). ..

    Article Title: Individual herpes simplex virus 1 (HSV-1) particles exit by exocytosis and accumulate at preferential egress sites.
    Article Snippet: .. HSV-1 amplicon vector construction and propagation The amplicon vector plasmid pCPD-HSV-N-EmGFP-DEST was constructed by the DNASU Plasmid Repository (Biodesign Institute, Arizona State University) as follows: the plasmid HSV-DYN-hM4Di was a gift from John Neumaier (Addgene plasmid # 53327) (79). ..

    Article Title: The Rab6 post-Golgi secretory pathway contributes to herpes simplex virus 1 (HSV-1) egress
    Article Snippet: .. The amplicon vector plasmid pCPD-HSV-N-EmGFP-DEST was constructed by the DNASU Plasmid Repository (Biodesign Institute, Arizona State University), as follows: the plasmid HSV-DYN-hM4Di was a gift from John Neumaier (Addgene plasmid #53327) ( ). ..

    Construct:

    Article Title: LIVE-CELL FLUORESCENCE MICROSCOPY OF HSV-1 CELLULAR EGRESS BY EXOCYTOSIS
    Article Snippet: .. The amplicon vector plasmid pCPD-HSV-N-EmGFP-DEST was constructed by the DNASU Plasmid Repository (Biodesign Institute, Arizona State University), as follows: The plasmid HSV-DYN-hM4Di was a gift from John Neumaier (Addgene plasmid # 53327) [ ]. ..

    Article Title: Herpes Simplex Virus 1 (HSV-1) Uses the Rab6 Post-Golgi Secretory Pathway For Viral Egress
    Article Snippet: .. The amplicon vector plasmid pCPD-HSV-N-EmGFP-DEST was constructed by the DNASU Plasmid Repository (Biodesign Institute, Arizona State University), as follows: The plasmid HSV-DYN-hM4Di was a gift from John Neumaier (Addgene plasmid # 53327) [ ]. ..

    Article Title: The Rab6 post-Golgi secretory pathway contributes to herpes simplex virus 1 (HSV-1) egress.
    Article Snippet: .. HSV-1 amplicon vector construction and propagation The amplicon vector plasmid pCPD-HSV-N-EmGFP-DEST was constructed by the DNASU Plasmid Repository (Biodesign Institute, Arizona State University), as follows: the plasmid HSV-DYN-hM4Di was a gift from John Neumaier (Addgene plasmid #53327) (47). ..

    Article Title: Individual herpes simplex virus 1 (HSV-1) particles exit by exocytosis and accumulate at preferential egress sites.
    Article Snippet: .. HSV-1 amplicon vector construction and propagation The amplicon vector plasmid pCPD-HSV-N-EmGFP-DEST was constructed by the DNASU Plasmid Repository (Biodesign Institute, Arizona State University) as follows: the plasmid HSV-DYN-hM4Di was a gift from John Neumaier (Addgene plasmid # 53327) (79). ..

    Article Title: The Rab6 post-Golgi secretory pathway contributes to herpes simplex virus 1 (HSV-1) egress
    Article Snippet: .. The amplicon vector plasmid pCPD-HSV-N-EmGFP-DEST was constructed by the DNASU Plasmid Repository (Biodesign Institute, Arizona State University), as follows: the plasmid HSV-DYN-hM4Di was a gift from John Neumaier (Addgene plasmid #53327) ( ). ..



    Similar Products

    plasmid hsv dyn hm4di  (Addgene inc)
    92
    Addgene inc plasmid hsv dyn hm4di
    Plasmid Hsv Dyn Hm4di, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+hsv+dyn+hm4di/HSV-DYN-hM4Di+(Plasmid+%2353327)/pmc11406995-195-20-28
    Average 92 stars, based on 1 article reviews
    plasmid hsv dyn hm4di - by Bioz Stars, 2026-11
    92/100 stars
    		  Buy from Supplier
    hm4di  (Addgene inc)
    92
    Addgene inc hm4di
    (a) schematic of dual-site viral injection approach using a retrograde HSV-EF1α-Cre-eYFP injected into the pPVN followed by a Cre-dependent AAV expressing <t>hM4Di-mCherry</t> or mCherry alone injected into vCA1. (b) representative image of co-localization between HSV-EF1α-Cre-eYFP and AAV-hSyn-DIO-hM4Di-mCherry in vCA1. Inset is magnified view of co-localization from panel B. (c) representative image of vCA1 mCherry + axons in the pPVN. (d) Schematic of 3-day auditory fear conditioning paradigm. Mice were administered Clozapine-N-oxide (CNO) 20 min prior to conditioning. (e) CNO had no effect in any group on baseline freezing to the conditioning context: (t 22 =0.110, p= 0.9126), (f) freezing during acquisition (t 22 =0.4393, p= 0.6647), or (g) baseline freezing in the novel CS testing context (t 22 =1.536, p= 0.1388). (h) hM4Di + CNO mice exhibited enhanced cue-specific freezing during non-reinforced exposure to conditioned CSs in a novel context, one-way (t 22 =4.467, p= 0.0002). (i) hm4di + CNO mice showed elevated freezing during non-reinforced re-exposure to the original conditioning context, (t 22 =4.212, p= 0.0004). (j) hM4Di + CNO mice exhibited enhanced shock responsivity to the first foot-shock during conditioning (Mann-Whitney 2-tailed U = 29.5, p = 0.0105), but not subsequent shocks (p > 0.05). (k) schematic of predator odor exposure paradigm highlighting early (1-5 min) and late phase (5-10 min) of the test. (l) hM4Di + CNO mice exhibited enhanced freezing during the first five minutes of exposure to the predator odor 2,5-dihydro-2,4,5-trimethylthiazoline (TMT), (t 22 =4.317, p= 0.0003). (m) hM4Di + CNO mice did not differ from other groups during the last five minutes of exposure to TMT, (t 22 =2.032, p= 0.0544). Bars and bar graph error bars are mean ± S.E.M. *p < 0.05, ***p < 0.001. conditioning: n = 12 mice/group, odors: n = 12 mice/group.
    Hm4di, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+hsv+dyn+hm4di/HSV-DYN-hM4Di+(Plasmid+%2353327)/bio_rxiv__2021__05__01__441648-172-26-28
    Average 92 stars, based on 1 article reviews
    hm4di - by Bioz Stars, 2026-11
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    (a) schematic of dual-site viral injection approach using a retrograde HSV-EF1α-Cre-eYFP injected into the pPVN followed by a Cre-dependent AAV expressing hM4Di-mCherry or mCherry alone injected into vCA1. (b) representative image of co-localization between HSV-EF1α-Cre-eYFP and AAV-hSyn-DIO-hM4Di-mCherry in vCA1. Inset is magnified view of co-localization from panel B. (c) representative image of vCA1 mCherry + axons in the pPVN. (d) Schematic of 3-day auditory fear conditioning paradigm. Mice were administered Clozapine-N-oxide (CNO) 20 min prior to conditioning. (e) CNO had no effect in any group on baseline freezing to the conditioning context: (t 22 =0.110, p= 0.9126), (f) freezing during acquisition (t 22 =0.4393, p= 0.6647), or (g) baseline freezing in the novel CS testing context (t 22 =1.536, p= 0.1388). (h) hM4Di + CNO mice exhibited enhanced cue-specific freezing during non-reinforced exposure to conditioned CSs in a novel context, one-way (t 22 =4.467, p= 0.0002). (i) hm4di + CNO mice showed elevated freezing during non-reinforced re-exposure to the original conditioning context, (t 22 =4.212, p= 0.0004). (j) hM4Di + CNO mice exhibited enhanced shock responsivity to the first foot-shock during conditioning (Mann-Whitney 2-tailed U = 29.5, p = 0.0105), but not subsequent shocks (p > 0.05). (k) schematic of predator odor exposure paradigm highlighting early (1-5 min) and late phase (5-10 min) of the test. (l) hM4Di + CNO mice exhibited enhanced freezing during the first five minutes of exposure to the predator odor 2,5-dihydro-2,4,5-trimethylthiazoline (TMT), (t 22 =4.317, p= 0.0003). (m) hM4Di + CNO mice did not differ from other groups during the last five minutes of exposure to TMT, (t 22 =2.032, p= 0.0544). Bars and bar graph error bars are mean ± S.E.M. *p < 0.05, ***p < 0.001. conditioning: n = 12 mice/group, odors: n = 12 mice/group.

    Journal: bioRxiv

    Article Title: A multisensory circuit for gating intense aversive experiences

    doi: 10.1101/2021.05.01.441648

    Figure Lengend Snippet: (a) schematic of dual-site viral injection approach using a retrograde HSV-EF1α-Cre-eYFP injected into the pPVN followed by a Cre-dependent AAV expressing hM4Di-mCherry or mCherry alone injected into vCA1. (b) representative image of co-localization between HSV-EF1α-Cre-eYFP and AAV-hSyn-DIO-hM4Di-mCherry in vCA1. Inset is magnified view of co-localization from panel B. (c) representative image of vCA1 mCherry + axons in the pPVN. (d) Schematic of 3-day auditory fear conditioning paradigm. Mice were administered Clozapine-N-oxide (CNO) 20 min prior to conditioning. (e) CNO had no effect in any group on baseline freezing to the conditioning context: (t 22 =0.110, p= 0.9126), (f) freezing during acquisition (t 22 =0.4393, p= 0.6647), or (g) baseline freezing in the novel CS testing context (t 22 =1.536, p= 0.1388). (h) hM4Di + CNO mice exhibited enhanced cue-specific freezing during non-reinforced exposure to conditioned CSs in a novel context, one-way (t 22 =4.467, p= 0.0002). (i) hm4di + CNO mice showed elevated freezing during non-reinforced re-exposure to the original conditioning context, (t 22 =4.212, p= 0.0004). (j) hM4Di + CNO mice exhibited enhanced shock responsivity to the first foot-shock during conditioning (Mann-Whitney 2-tailed U = 29.5, p = 0.0105), but not subsequent shocks (p > 0.05). (k) schematic of predator odor exposure paradigm highlighting early (1-5 min) and late phase (5-10 min) of the test. (l) hM4Di + CNO mice exhibited enhanced freezing during the first five minutes of exposure to the predator odor 2,5-dihydro-2,4,5-trimethylthiazoline (TMT), (t 22 =4.317, p= 0.0003). (m) hM4Di + CNO mice did not differ from other groups during the last five minutes of exposure to TMT, (t 22 =2.032, p= 0.0544). Bars and bar graph error bars are mean ± S.E.M. *p < 0.05, ***p < 0.001. conditioning: n = 12 mice/group, odors: n = 12 mice/group.

    Article Snippet: For experiments involving double injections, two weeks after the first injection, mice also received AAV-hSyn-flex-mGFP-2A-Synaptophysin-mRuby in vCA1 (Synaptotag: Stanford Vector Core), AAV2/8-Syn-dio-mCherry in vCA1 (control, Addgene), hM4Di (AAV2/8-Syn-DIO-hM4DI-mCherry; Addgene), AAV2/9-EF1α-dio-ChR2(E123A)-EYFP-WPRE-hGH (channelrhodopsin, Penn Vector Core), AAV2/9-EF1α-dio-EYFP-WPRE-hGH (control, Penn Vector Core).

    Techniques: Injection, Expressing, MANN-WHITNEY

    (a) truncated schematic representation of viral approach. (b) schematic of OF paradigm. (c-d) silencing has no effect on distanced traveled in the center (t 22 =1.234, p=0.2302) or periphery (t 22 =0.6727, p=0.5081) of the OF. (e-f) silencing has no effect on time spent in the center (t 22 =1.201, p=0.2424) or periphery (t 22 =1.237, p=0.2290) of the OF. (g) schematic of elevated plus maze. (h-j) silencing had no effect on time spent in the closed (t 13 =1.006, p=0.3326), open (t 13 =1.256, p=0.2313), or center areas (t 13 =0.1100, p=0.9141). (k) silencing had no effect on total distance traveled (t 13 =0.2184, p=0.8305) in the EPM. (l) schematic of PPI paradigm. (m) silencing had no effect on startle responsivity to tones alone (ANOVA 2-way Startle dB : F 1.623,42.21 = 57.15, p<0.0001). (n) silencing preferentially disrupted the highest intensity pre-pulse (ANOVA 2-way Time X Group interaction : F 2,51 = 19.61, p=0.0011). (o) silencing had no effect on short-term habituation (t 26 =0.8176, p=0.4210). Bar graph are mean ± S.E.M, Open Field: n mCherry =12 n hm4di =12, EPM: n mCherry =7 n hm4di =8, Startle: n mCherry =14 n hm4di =14, *p < 0.05.

    Journal: bioRxiv

    Article Title: A multisensory circuit for gating intense aversive experiences

    doi: 10.1101/2021.05.01.441648

    Figure Lengend Snippet: (a) truncated schematic representation of viral approach. (b) schematic of OF paradigm. (c-d) silencing has no effect on distanced traveled in the center (t 22 =1.234, p=0.2302) or periphery (t 22 =0.6727, p=0.5081) of the OF. (e-f) silencing has no effect on time spent in the center (t 22 =1.201, p=0.2424) or periphery (t 22 =1.237, p=0.2290) of the OF. (g) schematic of elevated plus maze. (h-j) silencing had no effect on time spent in the closed (t 13 =1.006, p=0.3326), open (t 13 =1.256, p=0.2313), or center areas (t 13 =0.1100, p=0.9141). (k) silencing had no effect on total distance traveled (t 13 =0.2184, p=0.8305) in the EPM. (l) schematic of PPI paradigm. (m) silencing had no effect on startle responsivity to tones alone (ANOVA 2-way Startle dB : F 1.623,42.21 = 57.15, p<0.0001). (n) silencing preferentially disrupted the highest intensity pre-pulse (ANOVA 2-way Time X Group interaction : F 2,51 = 19.61, p=0.0011). (o) silencing had no effect on short-term habituation (t 26 =0.8176, p=0.4210). Bar graph are mean ± S.E.M, Open Field: n mCherry =12 n hm4di =12, EPM: n mCherry =7 n hm4di =8, Startle: n mCherry =14 n hm4di =14, *p < 0.05.

    Article Snippet: For experiments involving double injections, two weeks after the first injection, mice also received AAV-hSyn-flex-mGFP-2A-Synaptophysin-mRuby in vCA1 (Synaptotag: Stanford Vector Core), AAV2/8-Syn-dio-mCherry in vCA1 (control, Addgene), hM4Di (AAV2/8-Syn-DIO-hM4DI-mCherry; Addgene), AAV2/9-EF1α-dio-ChR2(E123A)-EYFP-WPRE-hGH (channelrhodopsin, Penn Vector Core), AAV2/9-EF1α-dio-EYFP-WPRE-hGH (control, Penn Vector Core).

    Techniques: